Genome Editing in Mice Using TALE Nucleases.
نویسندگان
چکیده
Gene engineering for generating targeted mouse mutants is a key technology for biomedical research. Using TALENs as sequence-specific nucleases to induce targeted double-strand breaks, the mouse genome can be directly modified in zygotes in a single step without the need for embryonic stem cells. By embryo microinjection of TALEN mRNAs and targeting vectors, knockout and knock-in alleles can be generated fast and efficiently. In this chapter we provide protocols for the application of TALENs in mouse zygotes.
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عنوان ژورنال:
- Methods in molecular biology
دوره 1338 شماره
صفحات -
تاریخ انتشار 2016